Crystal Score Sheet Hr2 112 Crystal Screen 2 contains forty eight unique reagents To determine the formulation of each reagent simply read across the page Buffer pH is that of a 1 0 M 0 5 M for MES monohydrate stock prior to dilution with other reagent components pH with HCl or NaOH
HR2 112 Date Sample Reservoir Volume Drop Temperature 1 Clear Drop Buffer 2 Phase Separation 3 Regular Granular Precipitate 4 Birefringent Precipitate 5 Spherulites 6 Needles 1D 7 Plates 2D 8 Xtal 0 2 mm Crystal Screen 2 HR2 112 Scoring Sheet 1 10 PEG 6000 2 0 M Sodium Chloride 2 0 5 M Sodium Chloride 0 01 M CTAB Crystal Screen 2 is a straightforward effective and practical kit for deter mining preliminary crystallization conditions Crystal Screen 2 is also ef fective in determining the solubility of a macromolecule in a wide range of precipitants and pH
Crystal Score Sheet Hr2 112
Crystal Score Sheet Hr2 112
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Crystal Screen is a complete reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules Crystal Screen is a straightforward effective and practical kit for determin ing preliminary crystallization Crystal Screen HTTM is a high throughput reagent kit designed to provide a rapid screening method for the crystallization of biological macromolecules The kit is straightforward effective and practical for the determination of preliminary crystallization conditions
8 8 Single Crystals 3D Growth 0 2 mm 9 Single Crystals 3D Growth 0 2 mm Crystal Screen 2 Cryo HR2 121 Scoring Sheet Date Date Date 1 1 6 M Sodium chloride 8 w v Polyethylene glycol 6 000 20 v v Glycerol 2 0 3 M Sodium chloride 0 006 M Magnesium chloride hexahydrate 0 006 M Hexadecyltrimethylammonium bromide 40 v v
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Shop Hampton Research HR2 112 Crystal Screen 2 10 ml tube format at Fishersci Sample Sample Buffer Reservoir Volume Drop Volume Total l Sample l Reservoir l Additive l Sample Concentration Date Temperature 1 Clear Drop 2 Phase Separation 3 Regular Granular Precipitate 4 Birefringent Precipitate or Microcrystals 5 Posettes or Spherulites 6 Needles 1D Growth 7 Plates 2D Growth 8 Single Crystals 3D Growth 0 2 mm
Small bipyramid crystals clear drop 3 needle shaped crystals in 1 white precipitate One may also employ a standard numerical scoring scheme Clear 0 Precipitate 1 Crystal 10 etc Figure 4 on page 3 shows typical examples of what one might observe in a crystallization experiment Interpreting Index Crystal Screen 2 Cryo is a biased sparse matrix reagent kit designed to provide a rapid screening method for the crystallization of biological mac romolecules in the presence of a cryoprotectant
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Crystal Screen 2 contains forty eight unique reagents To determine the formulation of each reagent simply read across the page Buffer pH is that of a 1 0 M 0 5 M for MES monohydrate stock prior to dilution with other reagent components pH with HCl or NaOH
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HR2 112 Date Sample Reservoir Volume Drop Temperature 1 Clear Drop Buffer 2 Phase Separation 3 Regular Granular Precipitate 4 Birefringent Precipitate 5 Spherulites 6 Needles 1D 7 Plates 2D 8 Xtal 0 2 mm Crystal Screen 2 HR2 112 Scoring Sheet 1 10 PEG 6000 2 0 M Sodium Chloride 2 0 5 M Sodium Chloride 0 01 M CTAB
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Crystal Score Sheet Hr2 112 - 8 Single Crystals 3D Growth 0 2 mm 9 Single Crystals 3D Growth 0 2 mm Crystal Screen 2 Cryo HR2 121 Scoring Sheet Date Date Date 1 1 6 M Sodium chloride 8 w v Polyethylene glycol 6 000 20 v v Glycerol 2 0 3 M Sodium chloride 0 006 M Magnesium chloride hexahydrate 0 006 M Hexadecyltrimethylammonium bromide 40 v v
