Dirty Jokes That End Clean Analytical characterization of a monoclonal antibody therapeutic reveals a three light chain species that is efficiently removed using hydrophobic interaction chromatography
Analytical characterization of a monoclonal antibody therapeutic reveals a three light chain species that is efficiently removed using hydrophobic interaction chromatography mAbs ESI TOF MS Bioanalyzer and SDS PAGE all demonstrated that MAb A SEC Peak 1 contains two size variants the MAb A with one extra light chain 2H3L and the MAb A with two extra
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Genentech researchers recently reported the thorough characterization of a mAb species containing variants with one and two additional light chains present at 0 2 when In a model monoclonal antibody we observed a size variant with an extra light chain noncovalently associated with the monomer later named as 1 2mer The interaction
In this work we report the characterization of a mAb size variant using SE HPLC coupled with multi angle light scattering MALS matrix assisted laser desorption ionization time of flight In particular efforts are devoted to characterizing oligomeric variants owing to their potential immunogenic nature We report here a mAb variant consisting of a canonical mAb monomer
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Monoclonal antibodies have been established as the largest product class of biopharmaceuticals Since extensive characterization is required for development and quality Electron spray ionization time of flight mass spectrometry ESI TOF MS microfluidics capillary electrophoresis and sodium dodecyl sulfate PAGE SDS PAGE results demonstrated that
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https://www.sciencedirect.com › science › article › pii
Analytical characterization of a monoclonal antibody therapeutic reveals a three light chain species that is efficiently removed using hydrophobic interaction chromatography
https://pmc.ncbi.nlm.nih.gov › articles
Analytical characterization of a monoclonal antibody therapeutic reveals a three light chain species that is efficiently removed using hydrophobic interaction chromatography mAbs

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Dirty Jokes That End Clean - In particular efforts are devoted to characterizing oligomeric variants owing to their potential immunogenic nature We report here a mAb variant consisting of a canonical mAb monomer