Ms Round Pipe Section Weight Active Ras ELISA Activation Assay colorimetric format offers a sensitive and accurate dection of active Ras GTPase Additional assays for small GTPases including Rho Ras Cdc42 Rac
The Active Ras Detection Kit provides all reagents necessary for measuring activation of Ras GTPase in the cell GST Raf1 RBD fusion protein is used to bind the The assay uses the Ras binding domain RBD of the Ras effector kinase Raf1 The Raf RBD domain has been shown to bind specifically to the GTP bound form of Ras proteins The fact
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The main application of the assay is to identify compounds that lock KRAS in inactive OFF state by preventing GTP exchange Observation of an increase in HTRF signal upon binding of F GTP bound KRAS were subjected to in vitro kinase assay using purified Src kinase left lane and were separated into un phosphorylated KRAS mono phosphorylated
The KRAS gene is a member of the mammalian RAS gene family and encodes two different isoforms KRAS4A and KRAS4B through alternative splicing of the fourth exon The Direct targeting of the RAS GTP binding site has been dismissed as a therapeutic strategy in large measure because RAS has a high affinity for GTP and GDP nucleotides with
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Nonradioactive determination of Ras GTP levels using activated Ras interaction assay Methods Enzymol 333 333 48 Benard V and Bokoch G M 2002 Assay of Cdc42 Rac and Rho KRAS gp120GAP RAS GAP N His Tag GTP GDP KRAS SOS1 GDP GTP KRAS
The HTRF KRAS GTP binding assay is in a competitive assay format using a GTP Red reagent as KRAS WT ligand a 6His tagged human KRAS WT protein and an anti 6His Cryptate The Ras GTPase Chemi ELISA Kit is the first ELISA based kit designed to detect and When bound to GTP Ras is active and activates effector proteins involved in the downstream control
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Active Ras ELISA Activation Assay colorimetric format offers a sensitive and accurate dection of active Ras GTPase Additional assays for small GTPases including Rho Ras Cdc42 Rac
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The Active Ras Detection Kit provides all reagents necessary for measuring activation of Ras GTPase in the cell GST Raf1 RBD fusion protein is used to bind the
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Ms Round Pipe Section Weight - Figure 1 Schematic representation of the mechanism of the KRAS G12C Nucleotide Exchange Assay Kit KRAS is activated upon binding GTP and undergoes a conformational change
